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Verified Commit 953c2c15 authored by Marta Girona Alarcon's avatar Marta Girona Alarcon Committed by GitLab UZH
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...@@ -56,22 +56,25 @@ In the publication, we have shown the need of infusing contrast agent to achieve ...@@ -56,22 +56,25 @@ In the publication, we have shown the need of infusing contrast agent to achieve
![Fig. 2. Three dimensional rendering of CSF spaces enclosed in the mouse skull: Infusion of contrast agent in the lateral ventricles during high-resolution synchrotron radiation-based hard X-ray computed tomography imaging allows 3D visualization with virtual slicing in all directions. Lateral ventricles in blue, third ventricle in green, aqueduct in pink and fourth ventricle in yellow.](images/JP26-invivo-2scan-phase3-postinjection-brain-80-90min_web.png){width="600"} ![Fig. 2. Three dimensional rendering of CSF spaces enclosed in the mouse skull: Infusion of contrast agent in the lateral ventricles during high-resolution synchrotron radiation-based hard X-ray computed tomography imaging allows 3D visualization with virtual slicing in all directions. Lateral ventricles in blue, third ventricle in green, aqueduct in pink and fourth ventricle in yellow.](images/JP26-invivo-2scan-phase3-postinjection-brain-80-90min_web.png){width="600"}
### Timeseries movies
Movement of contrast agent over time for the presented timeseries can be observed in projections and reconstructions as movies under the folders "videos_Reconstructions.7z" and "videos_Projections.7z".
## Additional materials ## Additional materials
We have additively manufactured the mouse holder, optimized for vertical imaging while ensuring fixation of the mouse skull. We have additively manufactured the mouse holder, optimized for vertical imaging while ensuring fixation of the mouse skull (Figs. 3 and 4).
![Fig. 3. Visualization of the mouse holder mounted on the imaging rotational stage.](images/holder_1.jpeg) ![Fig. 3. Visualization of the mouse holder mounted on the imaging rotational stage.](images/holder_1.jpeg)
![Fig. 4. Transparent visualization of body mouse holder part containing the water channels to ensure physiological temperature of the mouse.](images/holder_2_holes.jpeg){width="300"} ![Fig. 4. Transparent visualization of body mouse holder part containing the water channels to ensure physiological temperature of the mouse.](images/holder_2_holes.jpeg){width="300"}
Additionally, we assambled a customized mouse stage for two surgical procedures: tracheotomy and cisterna magna infusion (Fig. ). Additionally, we assambled a customized mouse stage for two surgical procedures: tracheotomy and cisterna magna infusion (Fig. 5).
![Fig. 5. Customized tracheotomy and cisterna magna stage. Available as a 3D model in the downloads.](images/cisternamagna_stage.jpeg){width="450"} ![Fig. 5. Customized tracheotomy and cisterna magna stage. Available as a 3D model in the downloads.](images/cisternamagna_stage.jpeg){width="450"}
### ## Equipment and consumables
Equipment and consumables are provided as a table: The following table provides details of consumables, hardware and software that have been employed for this experiments.
```{r} ```{r}
tbl <- read.csv(file = 'Equipment_table.csv') tbl <- read.csv(file = 'Equipment_table.csv')
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