@@ -28,7 +28,7 @@ In this [DOI](https://doi.org/10.5281/zenodo.13773081), we provide full resoluti
### Pilot post mortem experiments
In a previous beamtime (ESRF, 2020, December), *ex vivo* imaging was performed. The goal of the pilot experiments was to chose a contrast agent for the presented *in vivo* experiments.
The list of used contrast agents is available under the Zenodo download entry "tables_exVivo". We considered the Barium-based contrast agent (CA) to be the most promissing one and therefore decided to employ it for the *in vivo* experiments (Fig. 1).
The list of used contrast agents is available under the Zenodo download entry "tables_exVivo.7z". We considered the Barium-based contrast agent (CA) to be the most promissing one and therefore decided to employ it for the *in vivo* experiments (Fig. 1).

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@@ -51,24 +51,21 @@ Table 1. List of *ex vivo* mouse number, including the injected site and contras
### CSF spaces segmentation
In the publication we have shown the need of infusing contrast agent
In the publication, we have shown the need of infusing contrast agent to achieve a semi-automatic segmentation. Here, we show a rendering of one segmentation. In the downloads, we have provided a segmentation of a timeseries imaging in the brain ventricles, which can be found under "videos_Reconstructions.7z".
![Fig. 2. Three dimensional rendering of CSF spaces enclosed in the mouse skull: Infusion of contrast agent in the lateral ventricles during high-resolution synchrotron radiation-based hard X-ray computed tomography imaging allows 3D visualization with virtual slicing in all directions. Lateral ventricles in blue, third ventricle in green, aqueduct in pink and fourth ventricle in yellow.]
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## Additional materials
We have additively manufactured the mouse holder, optimized for vertical imaging while ensuring fixation of the mouse skull.

![Fig. 3. Visualization of the mouse holder mounted on the imaging rotational stage.]

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![Fig. 4. Transparent visualization of body mouse holder part containing the water channels to ensure physiological temperature of the mouse.]
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Additionally, we assambled a customized mouse stage for two surgical procedures: tracheotomy and cisterna magna infusion (Fig. ).
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