@@ -32,6 +32,7 @@ In this [DOI](https://doi.org/10.5281/zenodo.13773081), we provide full resoluti
In a previous beamtime (ESRF, 2020, December), *ex vivo* imaging was performed. The goal of the pilot experiments was to chose a contrast agent for the presented *in vivo* experiments. The list of used contrast agents is available under the Zenodo download entry "tables_exVivo.7z". We considered the Barium-based contrast agent (CA) to be the most promissing one and therefore decided to employ it for the *in vivo* experiments (Fig. 1).
> Fig. 1. Ex vivo coronal slices of three different mice injected with (left) Mouse.number 23, Gadolinium-based, (middle) Mouse.number 19, Barium-based and (right) Mouse.number 30, Gold-based contrast agent.
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@@ -88,9 +89,9 @@ Movement of contrast agent over time for the presented timeseries can be observe
We have additively manufactured the mouse holder that is optimized for vertical imaging while ensuring fixation of the mouse skull (Fig. 4). As it can be seen in the right side, the body part of the holder has water channels that facilitate a good physiological temperature for the animal.
> Fig. 4. (Left) Visualization of the mouse holder mounted on the imaging rotational stage. (Right) Translucent visualization of body mouse holder part containing the water channels to ensure physiological temperature of the mouse.
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@@ -99,9 +100,9 @@ We have additively manufactured the mouse holder that is optimized for vertical
For the surgery room, we assambled a customized animal box to increase the starting body mouse temperature. We set the desired temperature to 33°C. Additionally, to facilitate the surgical interventions, we manufactured a mouse stage for tracheotomy and cisterna magna infusion (Fig. 5).
> Fig. 5. (Left) Customized prewarm box consisting of an anesthesia induction chamber attached to two reptile generic warming pads, which are connected to an automatic temperature controller. (Right) Customized tracheotomy and cisterna magna stage. Available as a 3D model in the downloads.
