@@ -31,7 +31,7 @@ In this [DOI](https://doi.org/10.5281/zenodo.13773081), we provide full resoluti
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@@ -31,7 +31,7 @@ In this [DOI](https://doi.org/10.5281/zenodo.13773081), we provide full resoluti
In a previous beamtime (ESRF, 2020, December), *ex vivo* imaging was performed. The goal of the pilot experiments was to chose a contrast agent for the presented *in vivo* experiments. The list of used contrast agents is available under the Zenodo download entry "tables_exVivo.7z". We considered the Barium-based contrast agent (CA) to be the most promissing one and therefore decided to employ it for the *in vivo* experiments (Fig. 1).
In a previous beamtime (ESRF, 2020, December), *ex vivo* imaging was performed. The goal of the pilot experiments was to chose a contrast agent for the presented *in vivo* experiments. The list of used contrast agents is available under the Zenodo download entry "tables_exVivo.7z". We considered the Barium-based contrast agent (CA) to be the most promissing one and therefore decided to employ it for the *in vivo* experiments (Fig. 1).
> Fig. 1. Ex vivo coronal slices of three different mice injected with (left) Mouse.number 23, Gadolinium-based, (middle) Mouse.number 19, Barium-based and (right) Mouse.number 30, Gold-based contrast agent.
> Fig. 1. Ex vivo coronal slices of three different mice injected with (left) Mouse.number 23, Gadolinium-based, (middle) Mouse.number 19, Barium-based and (right) Mouse.number 30, Gold-based contrast agent.
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@@ -91,7 +91,7 @@ We have additively manufactured the mouse holder that is optimized for vertical
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@@ -91,7 +91,7 @@ We have additively manufactured the mouse holder that is optimized for vertical
> Fig. 4. (Left) Visualization of the mouse holder mounted on the imaging rotational stage. (Right) Translucent visualization of body mouse holder part containing the water channels to ensure physiological temperature of the mouse.
> Fig. 4. (Left) Visualization of the mouse holder mounted on the imaging rotational stage. (Right) Translucent visualization of body mouse holder part containing the water channels to ensure physiological temperature of the mouse.
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@@ -100,7 +100,7 @@ We have additively manufactured the mouse holder that is optimized for vertical
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@@ -100,7 +100,7 @@ We have additively manufactured the mouse holder that is optimized for vertical
For the surgery room, we assambled a customized animal box to increase the starting body mouse temperature. We set the desired temperature to 33°C. Additionally, to facilitate the surgical interventions, we manufactured a mouse stage for tracheotomy and cisterna magna infusion (Fig. 5).
For the surgery room, we assambled a customized animal box to increase the starting body mouse temperature. We set the desired temperature to 33°C. Additionally, to facilitate the surgical interventions, we manufactured a mouse stage for tracheotomy and cisterna magna infusion (Fig. 5).