title: 'In vivo Imaging of Central Nervous System Fluid Spaces using Synchrotron Radiation Micro-Computed Tomography'
date: '2024-09-01'
First author: 'Marta Girona Alarcón'
Last author: 'Vartan Kurtcuoglu'
author:
- name: 'Marta Girona Alarcón (first author)'
orcid: 0009-0006-2111-2716
affiliations:
- name: The Interface Group, Institute of Physiology, University of Zurich
- name: 'Vartan Kurtcuoglu (last author)'
orcid: 0000-0003-2665-0995
affiliations:
- name: The Interface Group, Institute of Physiology, University of Zurich
format:
html:
grid:
body-width: 1500px
---
In this hub, we provide additional materials, methods and results for the publication.
## Supplemental Results
In this [DOI](https://doi.org/10.5281/zenodo.13773081), we provide full resolution datasets for download from the publication data and supplemental results:
### Pilot post mortem experiments
In a previous beamtime (ESRF, 2020, December), *ex vivo* imaging was performed. The goal of the pilot experiments was to chose a contrast agent for the presented *in vivo* experiments. The list of used contrast agents is available under the Zenodo download entry "tables_exVivo.7z". We considered the Barium-based contrast agent (CA) to be the most promissing one and therefore decided to employ it for the *in vivo* experiments (Fig. 1).
Table 1. List of *ex vivo* mouse number, including the injected site and contrast agent.
### CSF spaces segmentation
In the publication, we have shown the need of infusing contrast agent to achieve a semi-automatic segmentation. Here, we show a rendering of one segmentation. In the downloads, we have provided a segmentation of a timeseries imaging in the brain ventricles, which can be found under "videos_Reconstructions.7z".
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### Timeseries movies
Movement of contrast agent over time for the presented timeseries can be observed in projections and reconstructions as movies under the folders "videos_Reconstructions.7z" and "videos_Projections.7z" in [Zenodo](https://doi.org/10.5281/zenodo.13773081).
## Additional materials
We have additively manufactured the mouse holder, optimized for vertical imaging while ensuring fixation of the mouse skull (Fig. 3).
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Additionally, we assambled a customized mouse stage for two surgical procedures: tracheotomy and cisterna magna infusion (Fig. 5).
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## Equipment and consumables
The following table provides details of consumables, hardware and software that have been employed for this experiments.
