In the publication, we have shown the need of infusing contrast agent to achieve a semi-automatic segmentation. In the figure below we show a rendered segmentation. Infusion of contrast agent in the lateral ventricles during high-resolution synchrotron radiation-based hard X-ray computed tomography imaging allows 3D visualization with virtual slicing in all directions. In the downloads, we have provided a segmentation of a timeseries imaging in the brain ventricles, which can be found under "videos_Reconstructions.7z".
> Fig. 2. Three dimensional rendering of CSF spaces enclosed in the mouse skull. Lateral ventricles in blue, third ventricle in green, aqueduct in pink and fourth ventricle in yellow.