#!/bin/bash ## ## ## DATA=/home/imallona/src/polymenidou_manu_clip/01_snv_analysis BEDTOOLS=/home/imallona/soft/bedtools/bedtools-2.29.2/bin/bedtools WD=/home/imallona/src/polymenidou_manu_clip/03_postprocessing_snv_analysis # motif_sample=2000 fai=hg38.genome fasta=~/polymenidou_manu_clip/reference/GRCh38.primary_assembly.genome.fa DREME=~/soft/meme/bin/dreme STREME=~/soft/meme/bin/streme DUST=~/soft/meme/bin/dust ## to remove homopolymers cd $WD mkdir -p meme cd $_ mysql --user=genome --host=genome-mysql.cse.ucsc.edu -A -e \ "select chrom, size from hg38.chromInfo" > hg38.genome for fn in $(find $DATA -name "deletion_pos_min_3reads*.bed") do curr=$(basename $fn .bed) motif_sample=$(wc -l $fn | cut -f1 -d" ") # if [ "$motif_sample" -gt "3000" ]; then # motif_sample=3000 # fi motif_sample=2000 egrep "^chr[0-9XY]" $fn | \ $BEDTOOLS slop -s -l 10 -r 10 -i /dev/stdin -g $fai | \ shuf -n $motif_sample > "$curr"_peaks.bed $BEDTOOLS getfasta -s -fi $fasta -bed "$curr"_peaks.bed -fo "$curr"_peaks.fasta $DUST "$curr"_peaks.fasta > "$curr"_dusted.fasta $DREME -norc -o dreme_"$curr" -p "$curr"_dusted.fasta $STREME --p "$curr"_dusted.fasta \ -o streme_"$curr" \ --minw 3 \ --maxw 30 done # now for the clusters for fn in $(find $DATA -name "deletion_cluster_dist_10_min_3reads_*.bed") do curr=$(basename $fn .bed) motif_sample=$(wc -l $fn | cut -f1 -d" ") # if [ "$motif_sample" -gt "3000" ]; then # motif_sample=3000 # fi motif_sample=1000 egrep "^chr[0-9XY]" $fn | \ $BEDTOOLS slop -s -l 10 -r 10 -i /dev/stdin -g $fai | \ shuf -n $motif_sample > "$curr"_peaks.bed $BEDTOOLS getfasta -s -fi $fasta -bed "$curr"_peaks.bed -fo "$curr"_peaks.fasta $DUST "$curr"_peaks.fasta > "$curr"_dusted.fasta $DREME -norc -o dreme_"$curr" -p "$curr"_dusted.fasta $STREME --p "$curr"_dusted.fasta \ -o streme_"$curr" \ --minw 3 \ --maxw 30 done